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Fig. 2 | World Journal of Surgical Oncology

Fig. 2

From: miR-499a inhibits the proliferation and apoptosis of prostate cancer via targeting UBE2V2

Fig. 2

Role of UBE2V2 in the proliferation of prostate cancer cells. A WB analysis the expression of UBE2V2 protein in PC3 cells after transfection with control plasmid, UBE2V2 plasmid, control siRNA, or UBE2V2 siRNA. B Quantify the protein bands of the UBE2V2 protein (O.D. ratio over GAPDH). C Cell viability of PC3 cells was measured by MTT assay. PC3 cells were transfected with control plasmid, UBE2V2 plasmid, control siRNA, UBE2V2 siRNA, or co-transfected with UBE2V2 siRNA and UBE2V2 plasmid. D Colony formation assay of cell proliferation after PC3 cells were transfected with control plasmid, UBE2V2 plasmid, control siRNA, UBE2V2 siRNA, or co-transfected with UBE2V2 siRNA and UBE2V2 plasmid. Data are shown as mean ± SEM (n = 3). Asterisks indicate significant differences from the control (*, p < 0.05; **, p < 0.01; ***, p < 0.001, Student’s t test)

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