Fig. 6

SKIL could reverse the biological functions mediated by miR-216b-5p in CRC cells. A–D SKIL mRNA and protein levels in HCT-116 and SW480 cells transfected with miR-NC, miR-216b-5p, and miR-216b-5p + vector or miR-216b-5p + SKIL were examined using qRT-PCR and western blot, respectively. E–J The proliferation, apoptosis rate, migration, and invasion of transfected HCT-116 and SW480 cells were assessed by colony formation, flow cytometry, and Transwell assays, respectively. K, L The levels of E-cadherin, N-cadherin, and Vimentin in transfected HCT-116 and SW480 cells were measured by western blot. M–P Glucose consumption and lactate production of transfected HCT-116 and SW480 cells were measured by a glucose and lactate assay kit. Q, R The protein level of HK in transfected HCT-116 and SW480 cells was also measured by western blot. *P<0.05