Fig. 5

Circ_0029803 positively modulated SKIL expression via sponging miR-216b-5p. A The binding sites between miR-216b-5p and SKIL were predicted by StarBase v2.0. B, C The luciferase activity was assessed in HCT-116 and SW480 cells co-transfected with SKIL 3′ UTR WT or SKIL 3′ UTR MUT and miR-216b-5p or miR-NC by dual-luciferase reporter assay. D–G SKIL mRNA and protein levels in CRC tissues and cells compared with normal tissues and cells were detected by qRT-PCR and western blot, respectively. H–K The mRNA and protein levels of SKIL in HCT-116 and SW480 cells transfected with miR-NC or miR-216b-5p were examined by qRT-PCR and western blot, respectively. L–O The mRNA and protein levels of SKIL in HCT-116 and SW480 cells transfected with si-NC, si-circ_0029803, si-circ_0029803 + anti-miR-NC, or si-circ_0029803 + anti-miR-216b-5p were examined by qRT-PCR and western blot, respectively. *P<0.05