Fig. 2

Circ_0029803 knockdown suppressed the proliferation, migration, invasion, EMT, and glycolysis of CRC cells in vitro. After HCT-116 and SW480 cells were transfected with si-NC or si-circ_0029803. A, B Circ_0029803 expression was measured by qRT-PCR. C The cells proliferation was assessed by colony formation assay. D, E The cells apoptosis rate was determined by flow cytometry. F, G The migration and invasion of cells were detected by Transwell assay. H, I The protein levels of E-cadherin, N-cadherin, and Vimentin were checked by western blot. J–M Glucose consumption and lactate production of cells were measured by a glucose and lactate assay kit. N, O The protein level of HK was checked by western blot. *P<0.05