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Fig. 4 | World Journal of Surgical Oncology

Fig. 4

From: FoXA2 promotes esophageal squamous cell carcinoma progression by ZEB2 activation

Fig. 4

FOXA2 repressed ZEB2 expression transcriptionally. A ZEB2 expression was measured in ECA109 and YESE-140 cells transfection with si-FOXA2#1 by qRT-PCR and western blotting assays. B The luciferase activity of pGL3-ZEB2 reporter vector was detected in ECA109 and YESE-140 cells transfection with si-FOXA2#1. C The luciferase activity of pGL3-ZEB2 mutant vector was detected in ECA109 and YESE-140 cells transfection with si-FOXA2#1. D The binding of FOXA2 on the promoter of ZEB2 was confirmed by ChIP assay in ECA109 and YESE-140 cells. E The luciferase activity of pGL3-ZEB2 vector was detected in ECA109 and YESE-140 cells transfection with FOXA2 plasmids. F MMP9 expression was analyzed in ECA109 and YESE-140 cells transfection with si-FOXA2#1. *P < 0.05, **P < 0.01, ***P < 0.001

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