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Fig. 5 | World Journal of Surgical Oncology

Fig. 5

From: Identification of a novel circ_0018289/miR-183-5p/TMED5 regulatory network in cervical cancer development

Fig. 5

TMED5 was a direct target of miR-183-5p. A Sequence of miR-183-5p, the complementary sites for miR-183-5p in TMED5 3′UTR and the mutant of the seed sites. B Correlation between TMED5 level and the prognosis of patients with cervical cancer shown in the GEPIA database. C Dual-luciferase reporter assays using TMED5 3′UTR wild-type (TMED5 3′UTR-WT) and mutant (TMED5 3′UTR-MUT) reporter constructs in SiHa and HeLa cells. D, E TMED5 mRNA and protein levels in SiHa and HeLa cells transfected with anti-miR-NC, anti-miR-183-5p, miR-NC mimic, or miR-183-5p mimic by qRT-PCR and western blot assays, respectively. F, G TMED5 mRNA and protein levels in primary tumor samples and paired normal tissues from the same patients. H, I TMED5 mRNA and protein levels by qRT-PCR and western blot assays, respectively, in Ect1/E6E7, SiHa, and HeLa cells. J, K Correlation between TMED5 mRNA level and miR-183-5p expression or circ_0018289 expression in tumor samples analyzed by Pearson’s correlation coefficients. *P < 0.05

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