Fig. 4

Silencing of circ_0018289 impeded cervical carcinogenesis and angiogenesis by up-regulating miR-183-5p. A qRT-PCR analysis of miR-183-5p in SiHa and HeLa cells transfected with anti-miR-183-5p or anti-miR-NC. CCK-8 (B) and Edu (C) assays for cell proliferation, flow cytometry for cell apoptosis (D), western blot analysis for Bax and Bcl-2 levels (E), in SiHa and HeLa cells transfected with si-circ_0018289, si-NC, si-circ_0018289 + anti-miR-NC or si-circ_0018289 + anti-miR-183-5p. F Tube formation assay performed with human HUVECs pre-treated with the conditional medium of SiHa and HeLa cells transfected with si-circ_0018289, si-NC, si-circ_0018289 + anti-miR-NC or si-circ_0018289 + anti-miR-183-5p. G Western blot showing the levels of VEGFA and FGF2 in SiHa and HeLa cells transfected with si-circ_0018289, si-NC, si-circ_0018289 + anti-miR-NC or si-circ_0018289 + anti-miR-183-5p. *P < 0.05