Fig. 4

Sox4 and Twist1 are targets of miR-381-3p. A Schematic illustration of the predicted miR-381-3p binding sites in Sox4 and Twist1 3′-UTR. B, C Luciferase reporter assays were performed to demonstrate that miR-381-3p inhibits the wild-type (B), but not the mutant (C), 3′-UTR of Sox4 or Twist1 reporter activities. D The expression of Sox4 and Twist1 in MDA-MB-231 or SKBR3 cells transfected with miR-381-3p mimics or negative control was examined by western blot. E Cell viability in T47D cells transfected with miR-381-3p inhibitor or negative control with siRNAs targeting Sox4 or Twist1 was assessed by CCK-8 assay. F Colony formation analysis of T47D cells transfected with miR-381-3p inhibitor or negative control with siRNAs targeting Sox4 or Twist1. G Cell invasion in T47D cells transfected with miR-381-3p inhibitor or negative control with siRNAs targeting Sox4 or Twist1 was assessed by transwell analysis. *P < 0.05