Fig. 3
From: MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
MiR-186-3p directly targets IGF1. A Relative luciferase activity of the indicated IGF1 reporter construct in HeLa and SiHa cells is shown. ** P < 0.001 compared with miR-186-3p NC group using ANOVA. B RNA pull-down experiment showed that the biotinylated miR-186-3p in HeLa and SiHa successfully pulled down IGF1. ** P < 0.001 using Student’s t test. C The mRNA expression level of IGF1 in non-tumor or tumor tissue was detected by qRT-PCR. P < 0.001 using Student’s t test. D Pearson correlation analysis between the expression level of miR-186-3p and IGF1. E The relative expression of IGF1 in HcerEpic, HeLa, and CaSKi cells was measured by qRT-PCR. ** P < 0.001 compared with HcerEpic cells using ANOVA. NC, negative control; WT, wild-type; MUT, mutant