Fig. 5

MiR-330-5p directly targets NRBP1 in PCa cells. A The sequence of miR-330-5p binding sites within NRBP1. B Dual-luciferase reporter assay for the detection of the relative luciferase activities of wild and mutated NRBP1 reporter after miR-330-5p upregulation in 293T cells. C RIP assay for the examination of NRBP1 and miR-330-5p RNA enrichment in immunoprecipitation complexes in PC3 and DU145 cells. D Biotin-labeled pull-down assay indicating the interaction between NRBP1 and miR-330-5p in PC3 and DU145 cells. E, F The expression of NRBP1 in normal tissues, low-grade PCa, and high-grade PCa tissues was detected using qRT-PCR and western blot assay. G The expression of NRBP1 in PCa cells (PC3 and DU145) and normal RWPE-1 cells was determined with western blot. H NRBP1 expression was negatively correlated with miR-330-5p in PCa tissues. I Western blot analysis of NRBP1 expression in PC3 and DU145 cells transfected with si-NC, si-circLRP6#2, si-circLRP6#2 + inhibitor NC, or si-circLRP6#2 + miR-330-5p inhibitor. *P < 0.05