Fig. 4

Knockdown of circLRP6 suppresses PCa cell growth and metastasis via targeting miR-330-5p. A Analysis of the interference efficiency of miR-330-5p inhibitor or inhibitor NC using qRT-PCR. B–J PC3 and DU145 cells were transfected with si-NC, si-circLRP6#2, si-circLRP6#2 + inhibitor NC, or si-circLRP6#2 + miR-330-5p inhibitor. B qRT-PCR of miR-330-5p expression in cells. C–E Cell proliferation were determined by CCK-8 assay (C), colony formation assay (D), and EDU incorporation assay (E). F, G Flow cytometry for cell cycle and apoptosis. H Transwell assay for cell invasion. I Wound healing assay for cell migration. J Western blot analysis of the expression of E-cadherin and Vimentin. *P < 0.05