Fig. 3

MiR-330-5p is a target of circLRP6 in PCa cells. A The putative binding site between circLRP6 and miR-330-5p. B Dual-luciferase reporter assay for the relative luciferase activities of the wild and mutated circLRP6 reporter after miR-330-5p upregulation in 293T cells. C RIP assay for the examination of circLRP6 and miR-330-5p enrichment in immunoprecipitation complexes in PC3 and DU145 cells. D Biotin-labeled pull-down assay indicating the interaction between circLRP6 and miR-330-5p in PC3 and DU145 cells. E The expression of miR-330-5p in normal tissues, low-grade PCa and high-grade PCa tissues was detected using qRT-PCR. F The expression of miR-330-5p in PCa cells (PC3 and DU145) and normal RWPE-1 cells was determined with qRT-PCR. G MiR-330-5p expression was negatively correlated with circLRP6 in PCa tissues. *P < 0.05