Fig. 4

Mandatory overexpression of HIF-1 reversed the chemosensitization effect of miR-194-5p upregulation. a A CCK-8 cell viability test was performed to detect the survival ability of HIF-1-overexpressing A549 cells under different concentrations of DOX treatments. b TUNEL staining was used to detect the apoptosis rate of HIF-1-overexpressing A549 cells and control NSCLC cells (Bar=25 μm). c The expression levels of HIF-1, BAX, Caspase-9, Caspase-3, PARP, and p-gp in HIF-1-overexpressing NSCLC cells and miR-194-5p-overexpressing NSCLC cells induced by hypoxia were detected by western blot. Data are presented as the mean ± SD. *P <0.05; ***P < 0.001