Fig. 4

Correlation of XIST with miR-335 by direct targeting. A Wt-XIST 3′UTR luciferase reporter vector (wt-XIST) and mut-XIST 3′UTR luciferase reporter vector (mut-XIST) were obtained by successively mutating the calculated miR-335 combining site in XIST. B The luciferase activity of the wt-XIST luciferase reporter vector was significantly decreased in miR-335 cells. C The luciferase activity of the wt-XIST luciferase reporter vector was notably raised in the miR-335 inhibitor transfected cells. D XIST expression was upregulated in ovarian cancer tissues. E A negative correlation between XIST and miR-335 expressions in ovarian cancer tissues was identified. *P < 0.05, **P < 0.01