Fig. 2

Interaction of miR-335 with 3′UTR of BCL2L2. A The putative miR-335 binding site in its 3′UTR contained 5-bp mutant wt-BCL2L2 and corresponding mut-BCL2L2. B Wt-BCL2L2/mut-BCL2L2 vector and miR-335 NC/miR-335 mimic was co-transfected into SKOV3 and OVCAR3 cells. Compared with the control group, miR-335 mimic transfection significantly reduced the luciferase activity of the wt-BCL2L2 reporter gene. In co-transfected cells with miR-335 and mut-BCL2L2 reporter genes, no significant decrease in reporter gene activity was found. C Wt-BCL2L2/mut-BCL2L2 vector and miR-335 NC/miR-335 inhibitor were co-transfected into SKOV3 and OVCAR3 cells. The luciferase activity of the wt-BCL2L2 reporter gene was enhanced by the miR-335 inhibitor. ** P < 0.01