Fig. 7From: Hsa_circ_0001982 promotes the progression of breast cancer through miR-1287-5p/MUC19 axis under hypoxiaMiR-1287-5p directly interacted with MUC19 in BC cells. a Targetscan predicted the putative binding sites between circ_0001982 and miR-1287-5p. b, c The BC cells were co-transfected with MUC19 3′UTR-WT + miR-NC, MUC19 3′UTR-WT + miR-1287-5p, MUC19 3′UTR-MUT + miR-NC, and MUC19 3′UTR-MUT + miR-1287-5p. And the luciferase activity was measured. d–g The mRNA and protein levels of MUC19 were measured by RT-PCR and western blot in breast tissues and cells. h–k The mRNA and protein levels of MUC19 were measured by qRT-PCR and western blot assays in breast MDA-MB-231 and MDA-MB-468 cells under 48 h of hypoxia treatment. l–o The level of MUC19 was determined using the qRT-PCR and western blot analysis in MDA-MB-231 and MDA-MB-468 transfected with miR-1287-5p mimic under 48 h of hypoxia treatment. *P < 0.05Back to article page