Fig. 6

Downregulation of miR-1287-5p reversed the inhibitory effects of circ_0001982 knockdown on cell viability, migration, and invasion on progression of BC cells. The MDA-MB-231 and MDA-MB-468 cells in normal and hypoxia conditions were transfected with si-NC, or si-circ_0001982, si-circ_0001982 + anti-miR-NC, or si-circ_0001982 + anti-miR-1287-5p. a, b Cell activity was assessed by MTT assay in MDA-MB-231 and MDA-MB-468 cells under 48 h of hypoxia treatment. c, d The protein levels of cyclin D1 and PCNA in transfected cells were detected by western blot. e–h Transwell assay was used to investigate the migration and invasion capacities of MDA-MB-231 and MDA-MB-468 cells upon transfection. *P < 0.05