Fig. 3

Knockdown of circ_0001982 inhibited breast cancer cell viability, migration, and invasion in hypoxic conditions. MDA-MB-231 and MDA-MB-468 cells were transfected with si-NC or si-circ_0001982. a, b MTT was used to detect cell viability activity of cell lines (MDA-MB-231 and MDA-MB-468) under 48 h of hypoxia treatment. c, d Western blot assay was applied to test the protein levels of Cyclin D1 and PCN1 in MDA-MB-231 and MDA-MB-468 cells under 48 h of hypoxia treatment. e, f Transwell assay was employed to explore MDA-MB-231 and MDA-MB-468 cells migration and invasion abilities under 48 h of hypoxia treatment. *P < 0.05