Fig. 6

Circ_0055625 regulated MSI1 expression by binding to miR-338-3p. a The miRNAs associated with circ_0055625 and MSI1 were predicated by Starbase, Circinteractome, or Targetscan online database. b, c The binding sequences between miR-338-3p and circ_0055625 or MSI1 were predicted by Starbase online database and identified by Dual-Luciferase Reporter Assay. d The efficiency of miR-338-3p mimic and inhibitor in upregulating or downregulating miR-338-3p expression was detected by qRT-PCR. e The effect of circ_0055625 knockdown on miR-338-3p expression was investigated by qRT-PCR in SW480 and SW620 cells. f, g The impacts of miR-338-3p mimic or inhibitor on the mRNA and protein expression of MSI1 were determined by qRT-PCR and Western blot, respectively, in SW480 and SW620 cells. h, i The influences between circ_0055625 repression and miR-338-3p inhibitor on the mRNA and protein levels of MSI1 were determined by qRT-PCR and western blot, respectively, in SW480 and SW620 cells. **P<0.01 and ***P<0.001