Fig. 5

MSI1 overexpression impaired the inhibitory impacts of circ_0055625 knockdown on the progression and radioresistance of colon cancer. a, b The mRNA and protein expression of MSI1 were detected by qRT-PCR and Western blot, respectively, in SW480 and SW620 cells transfected with MSI1 or vector. c, d The effects between circ_0055625 silencing and MSI1 overexpression on the mRNA and protein expression of MSI1 were determined by qRT-PCR and Western blot, respectively, in SW480 and SW620 cells. e, g CCK-8 and colony formation assays were performed to disclose the effects between circ_0055625 silencing and enforced MSI1 expression on the proliferation of SW480 and SW620 cells. f The effects between circ_0055625 depletion and enforced MSI1 expression on the survival fraction of SW480 and SW620 cells under radiation treatment (0, 2, 4, and 6 Gy) were demonstrated by colony formation assay. h The influences between circ_0055625 absence and ectopic MSI1 expression on the apoptosis of SW480 and SW620 cells were unveiled by flow cytometry analysis. i, j Wound-healing and transwell migration assays were carried out to present the impacts between circ_0055625 silencing and MSI1 overexpression on the migration of SW480 and SW620 cells. k Transwell invasion assay was performed to present the impacts between circ_0055625 silencing and MSI1 overexpression on the invasion of SW480 and SW620 cells. **P<0.01 and ***P<0.001