Fig. 4

MSI1 knockdown inhibited tumorigenesis and radioresistance in colon cancer. a, b The mRNA and protein expression of MSI1 were severally detected by qRT-PCR and Western blot after si-MSI1#1, si-MSI1#2, or si-MSI1#3 transfection in SW480 and SW620 cells. c, d The effect of MSI1 silencing on the proliferation of SW480 and SW620 cells was revealed by CCK-8 assay. e, f Colony formation assay was conducted to study the effect of MSI1 repression on the survival fraction of SW480 and SW620 cells after treatment of radiation (0, 2, 4, and 6 Gy). g The effects of MSI1 knockdown on cell colony-forming ability with radiation presence or absence were elucidated by colony formation assay in SW480 and SW620 cells. h The impacts of MSI1 depletion on cell apoptosis with radiation presence or absence were presented by flow cytometry analysis in SW480 and SW620 cells. i, j Wound-healing and Transwell migration assays were performed to determine the impacts of MSI1 knockdown on the migration of SW480 and SW620 cells with radiation presence or absence. k Transwell invasion assay was performed to determine the impacts of MSI1 knockdown on the invasion of SW480 and SW620 cells with radiation presence or absence. *P<0.05, **P<0.01, and ***P<0.001