Fig. 5

UNC13C was a target of miR-96-5p. a, b RNA pull-down assay was used to assess the binding ability of miR-96-5p and 5 target genes (SDC2, UNC13C, REV1, CAMK2N1, and KIAAA2022). c The binding sites and mutant sites between miR-96-5p and UNC13C 3′UTR were shown. The interaction between miR-96-5p and UNC13C was assessed by dual-luciferase reporter assay d, e and RIP assay f, g. h The mRNA expression of UNC13C in 59 paired OSCC cancer tissues and adjacent normal tissues was examined by qRT-PCR. i WB analysis was used to detect UNC13C protein expression in 3 paired OSCC cancer tissues and adjacent normal tissues. j The correlation between miR-96-5p and UNC13C expression in OSCC cancer tissues was assessed by Pearson correlation analysis. k The protein expression of UNC13C in OSCC cells (HSC6 and OECM1) and HOK cells was determined using WB analysis. l The transfection efficiencies of miR-96-5p mimic and inhibitor were evaluated by measuring miR-96-5p expression using qRT-PCR. m HSC6 and OECM1 cells were transfected with miR-con, miR-96-5p, in-miR-con, or in-miR-96-5p. The protein expression of UNC13C was detected by WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001