Fig. 5

Hsa_circ_0000231 knockdown inhibits MYO6 expression by sponging miR-502-5p. a The binding sites between miR-502-5p and MY06 were predicted by targetScan online database. b The luciferase activities were detected by dual-luciferase reporter assay. c RIP assay was employed to explain the direct target between miR-502-5p and MYO6. d QRT-PCR explained that hsa_circ_0000231 regulated MYO6 expression by associating with miR-502-5p. e MYO6 mRNA expression was dramatically upregulated in CRC tissues compared with paracancerous normal tissues. f and i MYO6 protein expression level was significantly increased in CRC tissues and HCT116 and LoVo cells relative to control groups. g Pearson correlation analysis revealed that there was a poor relation between MYO6 level and hsa_circ_0000231 level. h MYO6 level was negatively correlated with miR-502-5p level. *P < 0.05