Fig. 3

Hsa_circ_0000231 acts as a sponge of miR-502-5p. a Circular RNA interactome online database predicted the binding sites between hsa_circ_0000231 and miR-502-5p. b The predicted binding sequence of miR-502-5p in hsa_circ_0000231 was verified by dual-luciferase reporter assay in both HCT116 and LoVo cells. c RIP assay revealed that the direct association between hsa_circ_0000231 and miR-502-5p in both HCT116 and LoVo cells. d QRT-PCR explained hsa_circ_0000231 overexpression obviously downregulated miR-502-5p expression, and hsa_circ_0000231 knockdown apparently upregulated miR-502-5p expression in both HCT116 and LoVo cells. e and g The expression level of miR-502-5p was obviously decreased in CRC tissues and cells compared with control groups. f Hsa_circ_0000231 expression was negatively related to miR-502-5p expression. *P < 0.05