Table 1 Sequences of primers
RNA | Sequences of primer |
|---|---|
lncRNA NEAT1 | forward 5′-ATGCCACAACGCAGATTGAT-3′ reverse, 5′-CGAGAAACGCACAAGAAGG-3 ′[26] |
VEGF-A | forward 5′-AGGGTTTCGGGAACCAGAT-3′ reverse, 5′-CTGGCCTTGCACATTCCT-3 ′[27] |
E-cadherin | forward 5′-TACACTGCCCAGGAGCCAGA-3′, reverse 5′-TGGCACCAGTGTCCGGATTA-3 ′[28] |
N-cadherin | forward 5′-TCAGGCGTCTGTAGAGGCTT-3′, reverse 5′-ATGCACATCCTTCGATAAGACTG-3 ′[28] |
Vimentin | forward 5′-GACGCCATCAACACCGAGTT-3′, reverse 5′-CTTTGTCGTTGGTTAGCTGGT-3 ′[28] |
Snail | forward 5′-TCGGAAGCCTAACTACAGCGA-3′, reverse 5′-AGATGAGCATTGGCAGCGAG-3 ′[28] |
Notch1 | forward, 5′-ATGCAGAACAACAGGGAGGA-3′, reverse 5′-ACCAGGTTGTACTCGTCCAG-3′ |
Notch2 | forward 5′-AATGGAGGCTATGGCTGTGT-3′, reverse 5′-GGCACATGCAAGAGAAGGAG-3′ |
Jagged1 | forward, 5′-ACTTACCAGCCGTGTCTCAA-3′, reverse 5′-GCCTCGAGCACATTGACATT-3′ |
GAPDH | forward, 5′-GGTCTCCTCTGACTTCAACA-3′, reverse, 5′-GTGAGGGTCTCTCTCTTCCT-3 ′[26] |