Fig. 4

LncRNA NEAT1 accelerated proliferation and EMT and repressed apoptosis of OSCC cells through activating Notch signaling pathway. a Expressions of Notch1, Notch2, and Jagged1 with overexpressed NEAT1-2 and VEGF-A were evaluated by RT-qPCR normalized with GAPDH and the statistical test was comparing oeNEAT1-2 and oeNEAT1-2+oeVEGF-A to oeNC, **P < 0.05. b Cell viabilities were checked in TSCC1 cells with overexpressed NEAT-1, overexpressed VEGF-A and IMR-1 via CCK-8 with the statistical test was comparing oeNEAT1-2, oeNEAT1-2+oeVEGF-A, and oeNEAT1-2+oeVEGF-A+IMR-1 to oeNC, **P < 0.05. c Flow cytometry was performed to measure apoptosis rate of TSCC1 cell line with overexpressed NEAT1-2 and VEGF-A and added IMR-1 with the statistical test was comparing oeNEAT1-2, oeNEAT1-2+oeVEGF-A, and oeNEAT1-2+oeVEGF-A+IMR-1 to oeNC, **P < 0.05. d RNA levels of E-cadherin, N-cadherin, Vimentin, and Snail were detected with overexpressed NEAT1-2, overexpressed VEGF-A and IMR-1 using RT-qPCR with normalizing to GAPDH and the statistical test was comparing E-cadherin, N-cadherin, Vimentin, and Snail in oeNEAT1-2, oeNEAT1-2+oeVEGF-A, and oeNEAT1-2+oeVEGF-A+IMR-1 group to E-cadherin, N-cadherin, Vimentin, and Snail in oeNC group, **P < 0.05. Each experiment was repeated three times