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Table 2 Specific primers designed for QMF PCR

From: Investigation of the SPAG5 gene expression and amplification related to the NuMA mRNA levels in breast ductal carcinoma

Gene Ch. Sequence Length Tm GC% Product (bp)
BOD1L 4 F-AATGCCTCCGCTTTCAGGC 19 61.05 57 151
R-ATCACTTGGCAACTCACACATGG 23 61.62 47
AGBL2 11 F-GCGAGCTGCATTCCATGCG 19 62.8 63 197
R-TCCCAGCTTTGGAAACGCAC 20 61.45 55
POR 7 F-AGCCACTTTGTGCCAGATCA 20 59.89 50 258
R-TCCAGCACGTGTTCACATCA 20 59.89 50
SPAG5-5′ 17 F-CCCTAAGAAGCCCAAAATGCG 21 59.57 52 119
R-TCCTGGAAAGTTGGGTCGAG 20 59.31 55
SPAG5-Middle F-AGGCTGCTCATCTGATTCATGC 22 61.07 50 394
R-CAAGCTACCATCTGCCCACG 20 61.09 60
SPAG5-3′ F-GTTAGGAAAGGGTCGAAAGGGC 22 61.2 54 451
R-ACACCCTATCAAAAGTCTGTTCC 23 58.35 43
  1. QMF PCR quantitative multiplex fluorescent polymerase chain reaction, Ch chromosome, Tm melting temperature, GC guanine cytosine, bp base pair, BOD1L biorientation of chromosomes in cell division 1 like 1, AGBL2 ATP/GTP-binding protein like 2, POR cytochrome P450 oxidoreductase, SPAG5 sperm-associated antigen