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Table 2 Specific primers designed for QMF PCR

From: Investigation of the SPAG5 gene expression and amplification related to the NuMA mRNA levels in breast ductal carcinoma

Gene

Ch.

Sequence

Length

Tm

GC%

Product (bp)

BOD1L

4

F-AATGCCTCCGCTTTCAGGC

19

61.05

57

151

R-ATCACTTGGCAACTCACACATGG

23

61.62

47

AGBL2

11

F-GCGAGCTGCATTCCATGCG

19

62.8

63

197

R-TCCCAGCTTTGGAAACGCAC

20

61.45

55

POR

7

F-AGCCACTTTGTGCCAGATCA

20

59.89

50

258

R-TCCAGCACGTGTTCACATCA

20

59.89

50

SPAG5-5′

17

F-CCCTAAGAAGCCCAAAATGCG

21

59.57

52

119

R-TCCTGGAAAGTTGGGTCGAG

20

59.31

55

SPAG5-Middle

F-AGGCTGCTCATCTGATTCATGC

22

61.07

50

394

R-CAAGCTACCATCTGCCCACG

20

61.09

60

SPAG5-3′

F-GTTAGGAAAGGGTCGAAAGGGC

22

61.2

54

451

R-ACACCCTATCAAAAGTCTGTTCC

23

58.35

43

  1. QMF PCR quantitative multiplex fluorescent polymerase chain reaction, Ch chromosome, Tm melting temperature, GC guanine cytosine, bp base pair, BOD1L biorientation of chromosomes in cell division 1 like 1, AGBL2 ATP/GTP-binding protein like 2, POR cytochrome P450 oxidoreductase, SPAG5 sperm-associated antigen