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Table 1 Specific primers designed for quantitative real-time PCR

From: Investigation of the SPAG5 gene expression and amplification related to the NuMA mRNA levels in breast ductal carcinoma

Gene Sequence (5′_3′) Length Tm GC% Product (bp)
PUM1 F-CCTACCAACTCATGGTGGATGT 22 59.76 50 83
R-AGCCAGCTTCTGTTCAAGACT 21 59.58 47
SPAG5 F-AAGGAGAAGACTGAACAAGAGACC 24 59.96 45.8 119
R-TCATCTGCCACTGCTGTCAAG 21 60.61 52.4
NUMA F-AGAGAGCAAACTAAGCAGGTGG 22 60.29 50 97
R-CCTGGACAGCCTTCAGCTTCT 21 62.06 57.1
  1. PCR polymerase chain reaction, Tm melting temperature, GC guanine cytosine, bp base pair, PUM1 pumilio RNA-binding family member 1, F forward, R reverse, SPAG5 sperm-associated antigen, NUMA nuclear mitotic apparatus protein