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Table 1 Specific primers designed for quantitative real-time PCR

From: Investigation of the SPAG5 gene expression and amplification related to the NuMA mRNA levels in breast ductal carcinoma

Gene

Sequence (5′_3′)

Length

Tm

GC%

Product (bp)

PUM1

F-CCTACCAACTCATGGTGGATGT

22

59.76

50

83

R-AGCCAGCTTCTGTTCAAGACT

21

59.58

47

SPAG5

F-AAGGAGAAGACTGAACAAGAGACC

24

59.96

45.8

119

R-TCATCTGCCACTGCTGTCAAG

21

60.61

52.4

NUMA

F-AGAGAGCAAACTAAGCAGGTGG

22

60.29

50

97

R-CCTGGACAGCCTTCAGCTTCT

21

62.06

57.1

  1. PCR polymerase chain reaction, Tm melting temperature, GC guanine cytosine, bp base pair, PUM1 pumilio RNA-binding family member 1, F forward, R reverse, SPAG5 sperm-associated antigen, NUMA nuclear mitotic apparatus protein