Fig. 4
From: MicroRNA-136-3p inhibits glioma tumorigenesis in vitro and in vivo by targeting KLF7
KLF7 is a downstream target of miR-136-3p. a The predicted binding sites of miR-136-3p on KLF7 3′ UTR using TargetScan database. b qRT-PCR analysis of miR-136-3p post 48 h transfection in LN-229 and U251 cells, #p < 0.01, compared with miR-NC. c, d Luciferase reporter assay determination of luciferase activity of KLF7 3′ UTR after 48 h transfection with miR-NC, miR-136-3p mimic, or anti-miR-136-3p in LN-229 and U251, #p < 0.01, compared with miR-NC. e qRT-PCR analysis of miR-136-3p post 48 h transfection with miR-NC, miR-136-3p mimic, or anti-miR-136-3p in LN-229 and U251, #p < 0.01, compared with miR-NC. f, g Western blot analysis of KLF7 expression after 48 h transfection with miR-NC, miR-136-3p mimic, or anti-miR-136-3p in LN-229 and U251, #p < 0.01, compared with miR-NC. h The correlation of miR-136-3p and KLF7 in glioma tissues using Graphpad prism. i qRT-PCR analysis of KLF7 in adjacent tissues and in glioma tissues; #p < 0.01, compared with adjacent tissues