Fig. 2

Overexpression of miR-136-3p inhibits glioma cell proliferation and migration in LN-229 and U251 cells. a qRT-PCR analysis of miR-136-3p in LN-229 and U251 cells post 48 h transfection with miR-NC or miR-136-3p mimic; #p < 0.01, compared with miR-NC. b CCK-8 assay determination of proliferation of LN-229 and U251 cells after transfection with miR-NC or miR-136-3p mimic at the indicated time; #p < 0.01, compared with miR-NC. c, d Soft-agar colony formation assay analysis of glioma cell growth after 15 days transfection with miR-NC or miR-136-3p mimic; #p < 0.01, compared with miR-NC. e, f qRT-PCR analysis of cell growth-associated genes, including Cyclin A1 and PCNA, in LN-229 and U251 cells after 48 h transfection with miR-NC or miR-136-3p mimic; #p < 0.01, compared with miR-NC. g, h Trans-well assay analysis of migration ability of transfected LN-229 and U251 cells with miR-NC or miR-136-3p mimic; #p < 0.01, compared with miR-NC. i, j qRT-PCR analysis of cell migration-associated genes, including E-cadherin and N-cadherin, in LN-229 and U251 cells after 48 h transfection with miR-NC or miR-136-3p mimic; #p < 0.01, compared with miR-NC