Fig. 3

XIST directly binds to miR-140-5p. a Predicted binding site between miR-140-5p and XIST. b The luciferase reporter assay demonstrated that XIST directly targeted miR-140-5p in Y79 cells. c Expression of miR-140-5p was decreased in RB tissue. d Correlation analysis between XIST and miR-140-5p in RB tissues. e XIST notably inhibits miR-140-5p expression in Y79 cells. f Expression of miR-140-5p in Y79 cells transfected with miR-140-5p mimics or miR-140-5p mimics + pc-XIST. g, h Repressive effects of miR-140-5p on cell invasion and proliferation of Y79 cells were attenuated by XIST overexpression. *P < 0.05, **P < 0.01, ^#P < 0.05, and ^##P < 0.01, compared with the miR-NC, miR-140-5p+pc-NC, normal, or NC group