Fig. 3
From: Over-expression of Nav1.6 channels is associated with lymph node metastases in colorectal cancer
Immunohistochemistry for Nav1.5 and Nav1.6 in surgically isolated lymph nodes. a, b Nav1.5 is lowly expressed in both non-metastatic and metastatic lymph nodes (magnification 10 × 40). Nav1.6 is lowly expressed in non-metastatic lymph nodes (c) but highly expressed in metastatic lymph nodes (d) (magnification, 10 × 40). e Comparison of the average optical density of Nav1.5- and Nav1.6-positive regions in metastatic lymph nodes (data from five patients). At least 3200-fold fields of view were randomly selected for each slice in each group for screenshots. Image-Pro Plus 6.0 software was used to select the same brown color as the unified standard for judging all photos. Each photo was analyzed to obtain the cumulative optical density (IOD) of each photo and the area of the tissue (AREA) and obtain the average optical density value (average optical, AO value), AO = IOD/AREA, the greater the AO value, the higher the positive expression level. f, g Expression of Nav1.5 and Nav1.6 in different regions of lymph node. The lower left area of the figure was the non-tumor metastatic region and the right area was metastatic region. There was no significant difference in the expression of Nav1.5 between these two regions while Nav1.6 is lowly expressed in the non-metastatic region but highly expressed in the metastatic region