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Table 1 Antibodies and reagents used for immunostaining

From: Immunologic cellular characteristics of the tumour microenvironment of hepatocellular carcinoma drive patient outcomes

Antigen m/p Clone Species Company Secondary antibody Company Substrate Antigen retrieval
CD68 m PG-M1 Mouse Agilent anti-mouse-Ig/ peroxidase Vector DAB Tris/EDTA pH 9.0
CD163 m 10D6 Mouse Leica Biosystems, Newcastle Upon Tyne, UK anti-mouse-Ig/ peroxidase Vector DAB 10 mM citrate, pH 5.5
  1. DAB 3,3′-diaminobenzidine, m monoclonal, p polyclonal, TAM tumor-associated macrophage