Table 1 Antibodies and reagents used for immunostaining
Antigen | m/p | Clone | Species | Company | Secondary antibody | Company | Substrate | Antigen retrieval |
|---|---|---|---|---|---|---|---|---|
CD68 | m | PG-M1 | Mouse | Agilent | anti-mouse-Ig/ peroxidase | Vector | DAB | Tris/EDTA pH 9.0 |
CD163 | m | 10D6 | Mouse | Leica Biosystems, Newcastle Upon Tyne, UK | anti-mouse-Ig/ peroxidase | Vector | DAB | 10 mM citrate, pH 5.5 |