Fig. 1

MiR-218 was upregulated and negatively correlated with IDO1 by direct targeting on IDO1 in cervical cancer tissues and cells. a The mRNA levels of miR-218 were downregulated in cervical cancer specimens. b The survival rate of miR-218 low group was lower than that in miR-218 high group. c The mRNA levels of IDO1 were upregulated in cervical cancer specimens. d The survival rate of IDO1 high group was lower than that in miR-218 high group. e MiR-218 and IDO1 were well negatively correlated in cervical cancer tissues. f, g The mRNA levels of IDO1 (f) increased, while miR-218 decreased (g) in HeLa, SiHa, C-33, and Caski cervical cells. h, i The protein levels of IDO1 increased in HeLa, SiHa, C-33, and Caski cervical cells. j The target sequences of miR-218 in the 3′-UTR fragment of IDO1 were identified by TargetScan bioinformatics analysis. k The co-transfection of miR-218 and IDO1-3′-UTR resulted in a significant downregulation of the luciferase activity. *P < 0.05 and **P < 0.01 vs. cervical epithelial HcerEpic cells in f–i. **P < 0.01 vs. Cntl+IDO1-3′-UTR group in k