Fig. 3

In vitro experiment. a HE staining of liver tissues for the depth of coagulation necrosis and coagulation degeneration at different soaking times in dehydrated ethanol. × 40 magnification. b After being soaked in the alcohol, the liver cells emerged to shrivel. Several layer cells of the surface appeared spindle; their cytoplasm was decreased and stained deep (arrow). Some cells appeared to be necrosis, and nuclear condensation or apoptotic bodies in the apoptotic cells were observed with time last. Below the surface layer cells, there were several layers of degeneration cells. These cells had plenty of plasma as the swelling of cells. Therefore, these cells appeared more larger and paler than normal liver cells (star). × 100 magnification. c The depth of coagulation necrosis and degeneration increased as a function of the duration of soaking in dehydrated ethanol, for both tissue samples obtained from the cut hepatectomy surface and the capsule section of the liver