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Fig. 1 | World Journal of Surgical Oncology

Fig. 1

From: M2-polarized tumor-associated macrophages facilitated migration and epithelial-mesenchymal transition of HCC cells via the TLR4/STAT3 signaling pathway

Fig. 1

THP-1 cells were successfully differentiated into M2-polarized macrophages. a Images of THP-1 cultured under normal conditions (left) or with PMA (320 nM) for 6 h and subsequently cultured with IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 18 h (right) (× 200). b Flow cytometry analysis: normal THP-1 cells (left) and PMA + IL-4 + IL-13-treated THP-1 cells (right) exhibit significant differences in CD68 expression (a marker of macrophage differentiation). c M2 markers were detected in native and M2 macrophages using RT-PCR. Compared with native macrophages, M2-polarized macrophages exhibit the IL-12low, IL-23low, IL-10high, and TGF-βhigh phenotype

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