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Table 1 Sequences used in analysis of IDH1 mutation by pyrosequencing

From: IDH1 R132C mutation is detected in clear cell hepatocellular carcinoma by pyrosequencing

Forward primer

 

5′-GCTTGTGAGTGGATGGGTAAAA-3′

 

Reverse primer

 

5′-biotin-ATTGCCAACATGACTTACTTGATC-3′

 

Sequencing primer

 

5′-TGGGTAAAACCTATCATC-3′

 

Dispensation order

 

GATACGTAGCATGTCAT

 

Sequence to analyze

 

ATAGGTNGTCAT