Fig. 3

RBM5 inhibited proliferation and DNA synthesis in gliomas cells. Cells expressing RBM5 were seeded into 96-well plates and were counted for consecutive 5 days. To determine DNA synthesis, BrdU was added 2 h after cells were cultured and absorbance value at 450 nm was detected at 24 and 72 h, respectively. a The growth of U251 cells overexpressing RBM5. b DNA synthesis in U251 cells overexpressing RBM5. c The growth of SHG44 cells overexpressing RBM5. d DNA synthesis in SHG44 cells overexpressing RBM5. Each experiment was repeated in triplicates. *P < 0.05 was considered as statistically significant