Table 1 Primers used for reverse transcription and amplification of genes related to liver cancer stem cells
Gene | Primer sequence(5′→3′) |
|---|---|
miR-21 | F: ACTCAGCTGG TAGCTTATCAGACTGATG |
R: TGGTGTCGTGGAGTCG | |
U6 | F: CTCGCTTCGGCAGCACA |
R: AACGCTTCACGAATTTGCGT | |
CD13 | F: TTCAACATCACGCTTATCCACC |
R: AGTCGAACTCACTGACAATGAAG | |
Ep-CAM | F: ATAACCTGCTCTGAGCGAGTG |
R: TGCAGTCCGCAAACTTTTACTA | |
CD90 | F:ATGAAGGTCCTCTACTTATCCGC |
R: GCACTGTGACGTTCTGGGA | |
OCT4 | F: GTGTTCAGCCAAAAGACCATCT |
R: GGCCTGCATGAGGGTTTCT | |
GAPDH | F: CTGGGCTACACTGAGCACC |
R: AAGTGGTCGTTGAGGGCAATG |