Fig. 3

Downregulation of CXCR7 expression in SGC-7901 cells by transfection with CXCR7shRNA. a After G418 selection, the protein expression levels of CXCR7 were measured by Western blot using anti-CXCR7 antibody and β-actin as a loading control. The experiment was repeated three times with similar results. b Cellular RNA was harvested after G418 selection, and CXCR7 mRNA was measured using RT-PCR. GAPDH was used as a loading control