Skip to main content
Fig. 4 | World Journal of Surgical Oncology

Fig. 4

From: miR-582-5P induces colorectal cancer cell proliferation by targeting adenomatous polyposis coli

Fig. 4

miR-582-5P targets the 3′ UTR of APC mRNA directly. a Schematic representation of mature miR-582-5P sequence and miR-582-5P target sites of the 3′ UTR of APC mRNA (red) and the 3′ UTR of APC mutant mRNA containing three altered nucleotides (green) in the putative target site (APC-3′ UTR-mut). b Western blotting analysis of APC (300KD) expression in SW480 cells transfected with miR-582-5P or miR-582-5P-in as compared to NC cells. c Luciferase assay of pGL3-APC-3′ UTR reporter co-transfected with 10 or 50 nM miR-582-5P mimic, miR-582-5P-in, or miR-582-5P-mut (pGL3-APC-3′ UTR) in SW480 cells. d, e Real-time PCR (d) and western blotting (e) analysis of cyclin D1 (cyclin D1, 34KD) and c-MYC (c-MYC, 53KD) mRNA expression in SW480 cells transfected with miR-582-5P mimic or miR-582-5P-in. Bars represent the mean ± SD of three independent experiments (*P < 0.05)

Back to article page