Fig. 3
From: miR-582-5P induces colorectal cancer cell proliferation by targeting adenomatous polyposis coli
Downregulation of miR-582-5P inhibits CRC cell proliferation. a Real-time PCR analysis confirming that SW480 cells were successfully transfected with miR-582-5P-in or NC (negative control) RNA. b MTT assay demonstrating that miR-582-5P downregulation reduced SW480 cell proliferation. c Downregulation of miR-582-5P reduced the colony formation ability of SW480 cells. Shown are representative micrographs (left) and quantification (right) of crystal violet-stained cell colonies at 10 days after transfection. d Anchorage-independent growth assay demonstrating that miR-582-5P downregulation reduced the tumorigenicity of SW480 cells. Shown are representative micrographs (left) and quantification of colonies >0.1 mm (right). Bars represent the mean ± SD of three independent experiments (*P < 0.05)