Fig. 2
From: miR-582-5P induces colorectal cancer cell proliferation by targeting adenomatous polyposis coli
Ectopic miR-582-5P expression induces CRC cell proliferation. a Real-time PCR analysis confirming that SW480 cells were successfully transfected with miR-582-5P mimic or NC (negative control) RNA. b MTT assay analysis of the effects of ectopic miR-582-5P expression on CRC cell proliferation. c Ectopic overexpression of miR-582-5P promoted the colony formation ability of SW480 cells. Representative micrographs (left) and quantification (right) of crystal violet-stained cell colonies at 10 days after transfection. d Anchorage-independent growth assay demonstrating that ectopic overexpression of miR-582-5P promoted SW480 cell tumorigenicity. Shown are representative micrographs (left) and quantification of colonies >0.1 mm (right). Bars represent the mean ± SD of three independent experiments (*P < 0.05)