Fig. 1

Overexpression of RBM5 enhanced autophagic vacuoles formation in A549 cells. A549 cells were transfected with recombined GV287-RBM5 plasmids (RBM5 group), plasmids with scrambled control sequence (control group), or co-treated with RBM5 transfection and classic autophagy inhibitor 3-MA (RBM5 + 3-MA group). a RT-PCR analysis of the mRNA levels of RBM5 and GAPDH in A549 cells. b Quantification of RBM5 mRNA levels relative to GAPDH. c Western blot analysis of the protein expression levels of RBM5 and β-actin in A549 cells. d Quantification of RBM5 protein expression relative to β-actin. e Images of acridine orange (AO) staining under fluorescence microscope. f Images of Monodansylcadaverine (MDC) staining. g Images acquired by transmission electron microscope. Data were presented as mean ± SD for three replicate experiments. *P < 0.05, ***P < 0.001