Fig. 2

Effect of endostatin/cyclophosphamide treatment on the mRNA expression of NOTCH-3, JAG-1, and DLL-4. The graphs represent relative mRNA expression levels of notch receptors, ligands, and downstream target genes at different concentrations of endostatin (E), CTX (C), and endostatin + CTX (E + C). The legends Conc. 1, Conc. 2, and Conc. 3 represent the treatment concentrations of E, C, and E + C. E was treated at 15, 10, and 5 mg/kg/day, CTX at 20, 10, and 5 mg/kg/day, and E + C at 15 + 20, 15 + 10, and 15 + 5 mg/kg/day. a NOTCH-1 mRNA expression level. b NOTCH-3. c NOTCH-4. d JAG-1 mRNA expression rate is similar to notch receptor expression with a greater expression in C-treated samples and a lower expression in the E and E + C-treated samples. e DLL-4 mRNA expression levels were high in E and E + C-treated samples whereas low in the C-treated samples. f Hes-1 mRNA expression. g Hey-1 mRNA expression. One-way ANOVA was performed, and the values were significant with p < 0.05 vs. control