Fig. 1
Low level of Klotho is detected in the liver cancer cell lines. The liver cancer cells (5 × 105 cells/well) were plated into 6-well plate. After 8 h, the endogenous Klotho levels in a panel of four liver cancer cell lines were detected with RT-PCR (a) and western blotting analysis (b). L02 is an immortalized liver cell line, and GAPDH is used as an internal reference