Fig. 3
5-FU-resistant colon cancer cell line exposed to high 5-FU concentrations. a 5-FU-resistant HCT116 colon cancer cells (1 × 104) were seeded onto 24 wells and were incubated with 5-FU for 24 h. Cell death and apoptosis was detected by cytometry using Annexin-V-FLUOS staining Kit. After washing cancer cells, the staining cell number were counted at ×200 magnification. Left panel: The cells were photographed at ×200 magnification. Right panel ANNEXIN-V-FLUOS staining cells. b The number of stained cells. Each bar represents the mean number of stained cells; error bar is SD. *p < 0.01