Figure 1

Representative MSP results in tumor tissues and the expression of DNMT1 protein in esophageal squamous carcinoma tissue. (A) Representative MSP results in tumor tissues: 1, 2, 3, 4, 5, and 6 for tumor tissues; 1 and 2 represented unmethylation which was the demethylation positive control; 4, 5, and 6 embodied hypermethylation and was the methylation positive control. IVD, in vitro DNA methylation; u, unmethylation; m, methylation. (B-D) The expression of DNMT1 protein in esophageal squamous carcinoma tissue by immunohistochemistry results (SP× 400). (B) Weakly positive; (C) positive strongly positive; (E) normal esophageal tissue, negative control.