Figure 2

CCK-8 assay was performed to analyze the effect of miR-29a-3p on cell proliferation ability in GC cells. (A) The cell growth rates of BGC-miR-29a-3p-mimics (upper) and MCG-miR-29a-3p-mimics (bottom) were detected by the CCK-8 assay. miR-29a-3p significantly decreased cell growth rates (*P < 0.05; independent student’s t-test). (B) The cell growth rates of BGC-miR-29a-3p-inhibitor (upper) and MCG-miR-29a-inhibitor (bottom) were detected by the CCK assay. miR-29a-3p knockdown significantly increased cell growth rates (*P < 0.05; independent student’s t-test). (C) Expression of CDK2, CDK4, CDK6, and CyclinD1 were detected in BGC-823 or MCG-803 transfected with miR-29a-3p mimics (left)/inhibitor (right). β-actin was used as a loading control. OD, optical density.