Decreased expression of miR-29a-3p in GC specimens and cell lines. (A) qRT-PCR analysis showing miR-29a-3p mRNA level in GC tissues (n = 50). The value (defined as “fold difference”) indicated the ratio of the miR-29a-3p mRNA expression level (fold change) in GC tissues to the matched non-tumor tissues. Twenty- one out of fifty GC cases showed a decreased expression of miR-29a-3p (T < N). The bar chart showed the number of detected GC cases. (B) Scatter plots of miR-29a-3p fold change in GC tissues and their matched non-tumor tissues. In both panels, the lines indicated the mean ± SD. The level of miR-29a-3p mRNA was significantly lower in GC tumor tissues compared to the non-tumor counterparts (P = 0.012, Paired Student’s t-test). (C) Semi-quantification of miR-29a-3p expression (fold change) in GC cell lines by qRT-PCR. Data were presented as means ± SD from at least three separate experiments.